Journal: Frontiers in bioscience (Landmark edition)

Article Title: SMARCA2 and SMARCA4 Participate in DNA Damage Repair.

doi: 10.31083/j.fbl2907262

Figure Lengend Snippet: Fig. 3. Lacking the enzymatic activities of SMARCA2/4 extends the accumulation of γH2AX, Ring Finger Protein 8 (RNF8) and Breast cancer susceptibility gene 1 (BRCA1). HeLa cells were pre-treated with or without FHD286 (1 µM) or Compound 14 (1 µM)

Article Snippet: The following antibodies were purchased from their respective companies: anti-γH2AX (Novus, NB100-384), anti-RAD51 (Catalog Number: MA1-23271; Invitrogen, Carlsbad, CA, USA), anti-ring finger protein 8 (RNF8) (Catalog number: 14112-1-AP, Proteintech, Wuhan, Hubei, China), anti-breast cancer susceptibility gene 1 (BRCA1) (Catalog Number: NB100-404, Novus, Littleton, CO, USA), anti-SMARCA2 (Catalog number: HPA029981; Sigma, Louis, MO, USA).

Techniques:

Journal: Biomedicines

Article Title: Roux-en-Y Gastric Bypass Modulates AMPK, Autophagy and Inflammatory Response in Leukocytes of Obese Patients

doi: 10.3390/biomedicines10020430

Figure Lengend Snippet: Autophagy and mitophagy markers evaluated in leukocytes of obese patients at one year follow-up. Protein expression and representative western blot images of ( A ) Beclin 1 ( n = 21), ( B ) ATG5 ( n = 21), ( C ) LC3-I ( n = 19), ( D ) LC3-II ( n = 19), ( E ) NBR1 ( n = 16) and mitophagy marker ( G ) PINK1 ( n = 20). Fluorescence microscopy images (100×) of ( F ) TMRM ( n = 26) as an indicator of mitochondrial membrane potential. Data are expressed as mean + standard error, * p < 0.05 when compared using a two-sided paired Student’s t-test. ATG5, autophagy related 5; LC3, microtubule-associated protein light chain 3; NBR1, neighbour of Brca1; RFU, relative fluorescence units; TMRM, tetramethylrhodamine methyl ester; PINK1, serine/threonine kinase phosphatase and tensin homolog (PTEN)-induced putative kinase 1.

Article Snippet: After blocking the membranes for 1 h with 5% skimmed milk in TBS-T, the proteins of interest were detected by overnight blotting at 4oC with the following antibodies: monoclonal anti-autophagy related protein 5 (ATG5) and polyclonal anti-LC3A/B (Cell Signaling Technology, Danvers, MA, USA), monoclonal anti-Beclin 1, monoclonal anti-AMPKα1α2, monoclonal anti-AMPKα1 (phospho T183) α2 (phospho T172), monoclonal anti-ATF6, and polyclonal anti-MCP-1 (Abcam, Cambridge, UK), monoclonal anti-CHOP and monoclonal anti-NF-κB (Thermo Fisher Scientific, Waltham, MA, USA), polyclonal anti-PINK1 (Sigma-Aldrich, Inc., St. Louis, MO, USA), and polyclonal anti-neighbour of BRCA1 gene 1 (NBR1) (Proteintech, Rosemont, IL, USA).

Techniques: Expressing, Western Blot, Marker, Fluorescence, Microscopy, Membrane

Journal: Frontiers in bioscience (Landmark edition)

Article Title: SMARCA2 and SMARCA4 Participate in DNA Damage Repair.

doi: 10.31083/j.fbl2907262

Figure Lengend Snippet: Fig. 3. Lacking the enzymatic activities of SMARCA2/4 extends the accumulation of γH2AX, Ring Finger Protein 8 (RNF8) and Breast cancer susceptibility gene 1 (BRCA1). HeLa cells were pre-treated with or without FHD286 (1 µM) or Compound 14 (1 µM)

Article Snippet: The following antibodies were purchased from their respective companies: anti-γH2AX (Novus, NB100-384), anti-RAD51 (Catalog Number: MA1-23271; Invitrogen, Carlsbad, CA, USA), anti-ring finger protein 8 (RNF8) (Catalog number: 14112-1-AP, Proteintech, Wuhan, Hubei, China), anti-breast cancer susceptibility gene 1 (BRCA1) (Catalog Number: NB100-404, Novus, Littleton, CO, USA), anti-SMARCA2 (Catalog number: HPA029981; Sigma, Louis, MO, USA).

Techniques: